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it is passively secreted into the bile, mainly in the form of acylcarnitine esters (greater than 66% of total carnitine) and long-chain acylcarnitine esters, amounting to 3050% of the total bile carnitine [56]

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For one subject, the spectrum obtained at the second post-dose time point was of insufficient quality, and was omitted from analysis

This is the clearest illustration of why the third, glucagon-receptor arm matters: glucagon signaling drives hepatic fat mobilization in a way a dual GIP/GLP-1 agonist does not directly target

Samples were then precipitated with ice-cold MeOH, and supernatants were retained and evaporated overnight using a SpeedVac system at 37 C

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