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For the morphology of EPCs, double staining with fluorescein isothiocyanate-labeled Ulex europaeus agglutinin-1 (FITC-UEA-1, L9006, Sigma, USA) and 1,1-dioctadecyl-3,3,3,3-tetramethylindocarbocyanine perchlorate-labeled acetylated low-density lipoprotein (Dil-acLDL, L3484, Eugene, USA) were used to identify the EPCs

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Reaming: If the bowl is clogged with excess cake (carbon buildup), gently ream it back to a thinner layer

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