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16-HBE cells were cultured as adherent monolayers in Eagles minimum essential medium (MEM) supplemented with 10% heat-inactivated (56 C, 30 min) fetal bovine serum (FBS), 1% MEM (non-essential aminoacids, Euroclone), 2 mM L-glutamine, and gentamicin (250 g/ml)

Second, TSH, by its effect of proliferation of the thyroid follicular cells has been long implicated in formation of thyroid carcinoma

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