Oxidant signaling in vascular cell growth, death and survival: a review of the roles of reactive oxygen species in smooth muscle and endothelial cell mitogenic and apoptotic signaling
After 1060 min at 35 C for recovery, slices were transferred into oxygenated aCSF containing (in mM): 125 NaCl, 2.5 KCl, 1.25 NaH 2 PO 4 , 2 CaCl 2 , 1 MgCl 2 , 26 NaHCO 3 , 15 Sucrose, and 10 Glucose (pH 7.2, 325 mOsm) at room temperature for the rest of the day and individually transferred to a recording chamber continuously perfused at 2 mL/min with oxygenated aCSF
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It was found that the antibody did not react against uninfected leaf material (lane 1), however, a distinct band at the expected size of 17.5 kDa, representing the TMV CP was observed in WT TMV infected material (lane 2)