Age and lung matched NHBE cultures redifferentiated at the ALI were exposed to CS and then infected with 5 ng of HIV BaL (R5-tropic) or HIV IIIB strain (X4-tropic) HIV as described by us earlier 5
A typical cigarette provides around 10 to 15 puffs
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Koszowski B, Rosenberry ZR, Yi D, Stewart S, Pickworth WB
World J Microbiol Biotechnol 28(5):20772086