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Transient expression of recombinant NtMMP1 To produce recombinant NtMMP1 for functional analysis, the 5' and 3' cDNA sequences were amplified, joined in-frame by SOE-PCR [35] and inserted into the plant expression vector pTRAkt [36]

In addition, you need to specify if you are applying for a full on-premises, limited on-premises, off-premises, or brewery-public house license