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Upon 85% confluence, PBEC were starved overnight using Lonza Bronchial Epithelial Basal Medium supplemented with Bronchial Epithelial Cell Growth Medium singlequots (except gentamycin, bovine pituitary extract and epidermal growth factor) (Lonza), BSA (1.5 g/mL) and 1% penicillin/streptomycin (Gibco) (i.e., BEGM starvation)

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Many social smokers may think they are not addicted, but numerous studies show that these individuals are, in fact, simply suppressing the same cravings that chain smokers experience

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